Fig. 7. ROS generated by PDT inhibited the expression of pro-inflammatory factors. (A) Western blotting analysis of the expression levels of iNOS, IL-12, and TNF-α at 12 h after PDT (n = 10). (B) Secretion levels of iNOS, IL-12, and TNF-α in cell supernatants were analyzed by western blotting (n = 10). (C) Effect of NAC on the pro-inflammatory factors TNF-α and CD16/32 12 h after different treatments by fluorescence assay (scale bar: 50 µm) (n = 10). * P<0.05, ** P<0.01, *** P<0.001 vs control group, ^# P<0.05, ^## P<0.01, ^### P<0.001 vs PDT group.